實(shí)驗(yàn)?zāi)康?/strong>
研磨果蠅,更有效快速的提取核酸蛋白。
實(shí)驗(yàn)地點(diǎn):國(guó)家蛋白質(zhì)(上海)中心C座319
實(shí)驗(yàn)材料和器具
樣品:果蠅60只,大概60mg
儀器:全自動(dòng)樣品快速研磨儀(上海凈信,JXFSTPRP-24)
耗材:專(zhuān)用研磨管(上海凈信,2ml),研磨珠(上海凈信)
試劑:液氮若干,PBS
實(shí)驗(yàn)步驟
1.準(zhǔn)備好2ml(樣品量大的話(huà),可選5ml規(guī)格)不銹鋼研磨管,將研磨管和研磨球清洗干凈,并滅菌滅酶;
2.將需要研磨的組織樣品放入準(zhǔn)備好的研磨管中,加入少許研磨珠。蓋好蓋子;
3.取出已經(jīng)冷凍的適配器,把裝好樣品研磨管插入適配器中;
4.將適配器裝入凈信研磨儀中;
5.設(shè)定好參數(shù),啟動(dòng)儀器至研磨程序結(jié)束。
實(shí)驗(yàn)結(jié)果
Previous:冷凍研磨儀研磨破碎大腸桿菌——上海凈信
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