大腸埃希氏菌(Escherichia coli)通常被稱為大腸桿菌,是Escherich在1885年發(fā)現(xiàn)的,在相當(dāng)長的一段時間內(nèi),一直被當(dāng)作正常腸道菌群的組成部分,認(rèn)為是非致病菌。直到20世紀(jì)中葉,才認(rèn)識到一些特殊血清型的大腸桿菌對人和動物有病原性,尤其對嬰兒和幼畜(禽),常引起嚴(yán)重腹瀉和敗血癥,它是一種普通的原核生物,根據(jù)不同的生物學(xué)特性將致病性大腸桿菌分為6類:腸致病性大腸桿菌(EPEC)、腸產(chǎn)毒性大腸桿菌(ETEC)、腸侵襲性大腸桿菌(EIEC)、腸出血性大腸桿菌(EHEC)、腸黏附性大腸桿菌(EAEC)和彌散粘附性大腸桿菌(DAEC)。大腸桿菌屬于革蘭氏陰性細(xì)菌(G-)。
高密度發(fā)酵是現(xiàn)代發(fā)酵工程的一頂新技術(shù),能顯著提高大腸桿菌菌體和基因工程產(chǎn)品的產(chǎn)量。通過用凈信冷凍研磨儀,能快速,有效的對大腸桿菌進(jìn)行破壁處理,能更好的進(jìn)行下一步研究。
實驗步驟
步驟1:把發(fā)酵好的樣品放到離心管中,加入適量的研磨珠;
步驟2 :將樣本放入冷凍適配器中,放入凈信冷凍研磨儀上;
步驟3:設(shè)置參數(shù):研磨頻率X和研磨時間;開啟制冷模式,使樣品研磨的環(huán)境在特定的溫度下進(jìn)行,時間X分鐘就對大腸桿菌進(jìn)行完全破壁。
步驟4:從凈信冷凍研磨儀上取出離心管,放到離心機離心一下,便可輕松提取樣品進(jìn)行進(jìn)樣處理。
需了解具體步驟,請致電廠家!
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