人和動物的肝臟具有極強的再生能力,研究人員也通常用大鼠來做實驗從中去研究肝臟的再生能力。通過分離、純化再生肝中各種細胞,研究大鼠肝再生肝臟細胞增殖周期變化及體外細胞增殖周期。
大鼠肝70%切除后可在7-10天內基本恢復原有的體積和功能。從負調機制角度對肝再生進行研究,闡明調控肝再生終止和肝臟組織結構重塑的分子機制,不僅有助于全面認識肝臟再生,同時對肝臟疾病,尤其是肝臟腫瘤病理機制的認識和治療靶點的探索等都具有十分重要的意義。
杭州阿諾生物醫(yī)藥科技有限公司在樣品前處理這塊也在使用我們凈信研磨儀,這次他們的實驗對象是大鼠。
實驗地點:阿諾醫(yī)藥
實驗材料和器具:
樣品:大鼠浸泡保存的臟器
儀器:全自動快速研磨儀(上海凈信JX-24)
配件:24金屬適配器、氧化鋯珠、氧化鋯珠
JXFSTPRP?系列研磨機采用了特殊的上下及左右晃動三維一體的震動模式,樣品在空間呈8字形三維運動,通過研磨珠(氧化鋯、鋼珠、玻璃珠、陶瓷珠)的高頻往復振動、撞擊、剪切。快速的實現(xiàn)目的。使研磨的樣品具有更加充分、更均勻、樣品重復性更好、樣品之間沒有交叉污染。
實驗步驟:
1、將整塊的大鼠浸泡保存的臟器處理成黃豆大小以備研磨;
2、將樣品加入到無酶離心管中;
3.、加入若干顆氧化鋯珠或氧化鋯珠;
4、設定運行參數(shù)
5、啟動儀器至研磨程序結束,可得到如圖所示樣品。
Previous:凈信高能研磨儀在茶研究中的應用
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