實(shí)驗(yàn)背景
大、小鼠皮膚提蛋白目前是各高校動(dòng)物實(shí)驗(yàn)室的一項(xiàng)難題,目前客戶用勻漿器手動(dòng)研磨方法操作麻煩,溫度控制難度大,蛋白容易在研磨過(guò)程中因瞬時(shí)溫度和研磨顆粒較大,無(wú)法做出好的實(shí)驗(yàn)效果。上海凈信研磨儀根據(jù)客戶要求提供技術(shù)支持,解決華中科技大學(xué)生科院某實(shí)驗(yàn)室技術(shù)難題。
某生物實(shí)驗(yàn)交流網(wǎng)站帖子詢問(wèn)WB解決方案,無(wú)準(zhǔn)確回答解決方案。
實(shí)驗(yàn)解決方案:
1.儀器配置:上海凈信多樣品組織研磨儀一臺(tái),模塊一套。
研磨儀主機(jī)/5ml不銹鋼罐適配器
2.實(shí)驗(yàn)器材需求:適量大小保溫瓶一個(gè),眼科剪,鑷子,鑰勺,后續(xù)配套離心管、裂解液。
皮膚去脂肪層鋪展開(kāi)效果/ 取材,準(zhǔn)備放入適配器液氮冷凍
將皮膚放入5ml不銹鋼罐,剪碎至一定程度,放入鋼珠,然后一同放入保溫瓶中冷凍至不沸騰,大概3分鐘左右將適配器一同快速裝入研磨機(jī)中,調(diào)好頻率和時(shí)間研磨。研磨完成后將組織轉(zhuǎn)入EP管中用裂解液溶解。
皮膚研磨后效果圖/加入裂解液后效果圖
研磨完成后進(jìn)行體蛋白提取及后續(xù)實(shí)驗(yàn),此方法最小通量為24個(gè)2mlEP管。大大提高了實(shí)驗(yàn)人員的工作效率,并且研磨時(shí)間短并且均衡,能控制好全程溫度在-80℃以下。
總結(jié):上海凈信研磨儀對(duì)皮膚及動(dòng)、植物或各種材料標(biāo)本有全套解決方案,高通量地解決實(shí)驗(yàn)重難點(diǎn)問(wèn)題,讓實(shí)驗(yàn)更加輕松快捷,省去大量人力物力,深受各高校喜愛(ài)。
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