實驗目的
對擬南芥或水稻穗進行研磨破碎,進而提取其DNA。
實驗原理
通過研磨珠的撞擊,充分擬南芥或水稻穗細胞,使其內(nèi)容物流出,進而提取目標成分。
實驗材料和器具
樣品:擬南芥或水稻穗
儀器:全自動樣品冷凍研磨儀(上海凈信,Tissuelyser-48)
耗材:離心管,不銹鋼研磨珠(上海凈信)
試劑:TPS溶液(100mM Tris-Cl (pH 8.0),10mM EDTA (pH 8.0),1M KCl)
實驗步驟
需了解具體步驟,請致電廠家
實驗注意事項
1.-20攝氏度下該方法的DNA可以保存4個月以上,4°大致可以放2-3周。
Previous:tissuelyser組織研磨機對酵母菌研磨破壁方法
Related Products
- Home
- About us
-
Product
-
Grinder for scientific research
Grinder for scientific research
- Enhanced multi-sample tissue grinder
- Enhanced automatic sample rapid grinder
- Enhanced cryomill
- Multi-sample tissue grinding machine
- Automatic sample rapid grinder
- Cryogenic tissue grinder
- Three-dimensional motion grinder
- Single cell suspension preparation instrument
- Freezer tissue grinder
- Liquid nitrogen Cryogenic Grinder
- High Throughput Tissue Grinder
-
Grinder for analysis?and?detection
Grinder for analysis?and?detection
- Hair drug detector
- Handheld Grinder
- QuEChERS pre-processing all-in-one machine
- Soil/High Energy Planetary Grinder
- Hammer cyclone mill
- Knife grinder
- Multifunctional/Automatic Homogenizer
- Three dimensional vibrating ball mill
- Chlorophyll/soil A grinder
- Mortar grinder
- High-efficiency vibrating disc grinder
- Jaw grinder
- Flap homogenizer
- Bead-milling tissue grinder
- Special grinder for African swine fever
- Three-dimensional mixing instrument
- Miniature bead mill
- Forensic bone and tooth ball mill
- Percussion grinder
- Rotary splitter
-
General?Instruments
General?Instruments
- Ultrasonic cell disruptor
- Ultrasonic equipment
- In situ hybridization instrument/n instrument/crosslinking
- instrument Dry type thermostat/constant temperature mixer
- Vortex mixer/nitrogen purging instrument
- PCR instrument/membrane sealing instrument Snow ice
- Microplate constant temperature shaking incubator
- Orbital shaker/micro centrifuge
- Inoculation loop sterilization/indicator incubator
- Solid phase extraction device/low temperature thermostat
- Snow ice maker/viscometer/lyophilizer
- Flip oscillator
- Soil drying box/humidity box
-
Xiaomei Ultrasound Series
Xiaomei Ultrasound Series
-
Vibrating?sieve?shakers
Vibrating?sieve?shakers
-
Foreign distribution products
Foreign distribution products
-
Grinder for scientific research
- Customers
- News
- Videos
- Case
- Cantact