實驗?zāi)康?/strong>
通過對心肺組織的加藥及藥后的組織破碎檢測,計算組織的藥物代謝情況。
實驗原理
通過研磨珠撞擊心肺組織細胞,使其細胞膜破裂,再檢測其藥物殘留濃度。
實驗材料和器具
樣品:加藥后的心肺組織細胞
儀器:高通量組織研磨儀(上海凈信,Tissuelyser-48)
耗材:離心管,氧化鋯研磨珠(上海凈信)
實驗步驟
1.采集加藥后的心肺組織粗樣,分別放入研磨管中
2.管中加入氧化鋯珠,放入研磨儀中
3.研磨參數(shù)按要求設(shè)置,程序結(jié)束即可得到需要的破碎物。
實驗注意事項
1.在做定量分析時,破碎完的樣品直接離心,離心后吸取上清,研磨珠和雜質(zhì)沉淀于底部(棄去),檢測上清藥物濃度,得到更準確的藥物代謝數(shù)據(jù)。
2.若后續(xù)樣本需要做質(zhì)譜,研磨珠材質(zhì)只能選用氧化鋯,否則會影響數(shù)據(jù)。
Previous:高通量組織研磨儀研磨腫瘤組織提取RNA
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