實(shí)驗(yàn)?zāi)康?/strong>
對(duì)大腸桿菌進(jìn)行破壁,提取其蛋白質(zhì)。
實(shí)驗(yàn)原理
通過研磨珠的撞擊,破碎大腸桿菌的細(xì)胞壁,使其內(nèi)容物流出,進(jìn)而提取其蛋白質(zhì)。
實(shí)驗(yàn)材料和器具
樣品:大腸桿菌
儀器:全自動(dòng)樣品冷凍研磨儀(上海凈信,JXFSTPRP-96)
耗材:離心管,研磨珠(上海凈信)
實(shí)驗(yàn)步驟
需了解具體步驟,請(qǐng)致電廠家
實(shí)驗(yàn)結(jié)果
圖注:泳道3:marker
泳道2:0號(hào)對(duì)照
泳道1:含8M尿素上清
泳道0:bingding buffer 上清
泳道 0 1 2 3
本蛋白形成包涵體,在使用此研磨機(jī)后進(jìn)行上樣電泳。圖譜清晰,說明本研磨機(jī)適用于含包涵體菌體的破碎。
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