在生物科研、農(nóng)產(chǎn)品及種子檢驗(yàn)和進(jìn)出口商品檢驗(yàn)檢疫等領(lǐng)域,經(jīng)常需要對將植物組織進(jìn)行研磨以獲得DNA、RNA或蛋白質(zhì)等生物大分子,從而進(jìn)行后續(xù)的分析和檢測。隨著實(shí)驗(yàn)室科研進(jìn)度的日益加快,手動(dòng)研磨已無法滿足科研工作的需要,越來越多的科研人員都開始使用植物組織研磨器進(jìn)行研磨,不僅省時(shí)省力,也更簡單高效。其中,目前比較普及的研磨機(jī)器大都基于研磨鋼珠高速碰撞原理,最常見的主要有2大類,一類是凈信快速組織研磨儀,代表機(jī)型如某公司生產(chǎn)的Tissuelyser/JX-FSTPRP系列的自動(dòng)組織研磨器,另一類則是常規(guī)的研磨機(jī)器,比較有代表性的是德國某品牌組織研磨機(jī)。那么這2類機(jī)器有哪些性能特點(diǎn)呢?在使用上有無區(qū)別?研磨效果方面有哪些差異?來自實(shí)驗(yàn)的您,又該如何選擇機(jī)型呢?本文以上面2種代表機(jī)型做參考進(jìn)行比較,對這些問題做一個(gè)簡要總結(jié):
那么如何選擇適合自己的機(jī)型呢?
選擇常規(guī)研磨機(jī)理由:需要一次研磨處理大于200個(gè)微量樣品,或者一次處理超大體積樣品時(shí);不需要冷凍研磨。
選擇凈信快速組織研磨儀理由:習(xí)慣在實(shí)驗(yàn)室臺面上操作;一次處理量在200個(gè)微量樣品以下,同時(shí)需要研磨較大體積或質(zhì)地堅(jiān)硬的樣品;需要經(jīng)常進(jìn)行冷凍研磨;
關(guān)于價(jià)格方面,就目前了解到的信息,凈信快速植物組織研磨儀與國產(chǎn)常規(guī)的研磨機(jī)器的價(jià)格相差不大(后者均價(jià)略貴),而兩種都有不同型號配置的機(jī)器,所以定價(jià)不一,但市場價(jià)位一般在3-6萬左右,相比同類進(jìn)口機(jī)型動(dòng)輒10萬以上的高價(jià),實(shí)則優(yōu)惠較多。實(shí)際上,無論技術(shù)與質(zhì)量,目前國產(chǎn)和進(jìn)口研磨機(jī)器的差距已經(jīng)非常小,而在現(xiàn)貨以及售后方面,國產(chǎn)研磨機(jī)器則具有明顯優(yōu)勢,畢竟省去了漫長的貨期和高昂的維修費(fèi)是廣大用戶喜聞樂見的。而出于愛國主義情懷,筆者當(dāng)下也更傾向于選擇國產(chǎn)機(jī)器。當(dāng)然,最終選擇凈信快速組織研磨儀還是常規(guī)組織研磨器必須結(jié)合實(shí)驗(yàn)室的實(shí)際需求與具體預(yù)算綜合考慮了。
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